Figure 4. C11ORF24 is a transmembrane protein.
(A) HeLa cells expressing GFP-C11ORF24 (line 1) or GFP-GalT (line 2) were bleached in a 3,5µm circular region of the Golgi apparatus (red circle) and then imaged for 2 minutes by spinning disk microscopy. The fluorescence recovery after photobleaching was measured in 3 independent experiments and plotted for GFP-GalT (red, n=18 cells) and GFP-C11ORF24 (black, n=22 cells). Error bars indicate the standard deviation. The recovery was similar for the two proteins.