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. 2013 Sep 4;7:21. doi: 10.1186/1754-1611-7-21

Figure 2.

Figure 2

Sample raw data of the force-deformation responses obtained from atomic force microscopy (AFM). Only the approach curve was analyzed as it is the initial measurement of force responses in the compression test. The deformation at which the force amplitude was at least 2% of the maximum force was chosen to be the initial contact point between the cantilever and the axon (approximately 0.9 μm, gray area).