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. 2013 Dec 2;8(12):e81734. doi: 10.1371/journal.pone.0081734

Figure 6. TARSL2 as a member of ARS core complex.

Figure 6

(A, B) TARSL2 and TARS were detected in AIMP1, AIMP2, and KARS immunoprecipitates of HEK 293T (A) and HCT-8 cells (B). EPRS was used as a positive control. Actin was used for loading control. TCL; total cell lysate, SA pull down; streptavidin pull down. (C) Endogenous EPRS was co-immunoprecipitated with TARSL2. Cell lysate (500 μg) was immunoprecipitated with the antibodies against TARSL2, TARS, and IgG and probed for EPRS, TARSL2 and TARS. (D) Reciprocal co-immunoprecipitation. Cell lysate (500 μg) was immunoprecipitated with the antibodies against EPRS, AIMP1, AIMP2, KARS and IgG and probed for TARSL2. IgG was used for immunoprecipitation control. M; Mock, E; ERPS, A1; AIMP1, A2; AIMP2, K; KARS, In; Input, TL2; TARSL2, T; TARS, IgG(R); rabbit IgG, IgG(M); mouse IgG. Closed arrowheads (▶) indicate TARSL2 and TARS.