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. 2013 Dec 2;8(12):e81187. doi: 10.1371/journal.pone.0081187

Figure 2. Permeability is not altered during T. cruzi infection and TEM.

Figure 2

EC monolayers were incubated with T. cruzi identical to the standard TEM reaction except that 100 µg/mL FITC-dextran was included in the media to assay monolayer permeability. After a three hour incubation, samples were washed extensively and the amount of FITC-dextran that had crossed into the collagen matrix was quantified using a fluorescence spectrophotometer. Data shown represent the mean and standard deviation of six individual replicates. * denotes p<0.05 for the indicated comparisons.