Fig. 1.
DAXX interacts with trimeric HSF1 forms and stimulates basal HSF1 activity. (A) Interaction of HSF179–350 and DAXX560–740 sequences in yeast strain EGY48 grown on galactose-containing medium lacking leucine. AD, activation domain. (B) Interaction of DAXX and HSF1 in human cells. Two sets of cultures were transfected with 2 μg (per 60-mm dish) of expression construct for FLAG-tagged DAXX or FLAG vector. One day later, one set was heat-treated (HS). DAXX-containing complexes were immunoprecipitated from extracts by using FLAG antibody, and immunoprecipitates were analyzed by Western blot by using HSF1 antibody (Upper) or FLAG antibody (Lower). (C) Enhancement of basal HSF1 activity by overexpressed DAXX. Amounts of expression constructs cotransfected in addition to 25 ng of hsp70-fluc/Rluc reporter mixture are shown below. (Inset) Anti-FLAG Western blot signals produced by expression of DAXX (Upper) and DAXX625–740 (Lower).