Fig. 6.

Phosphorylation of pRB is rapidly induced in interscapular BAT but not in epididymal WAT upon cold exposure. C57BL/6 mice were preacclimated to 30°C for 2 weeks and then transferred to 4°C. Interscapular BAT and epididymal WAT were isolated at the indicated days after cold exposure and prepared for SDS/PAGE. Levels of UCP-1 expression, pRB phosphorylation, and total pRB were determined by Western blotting. P-pRB indicates Ser 780-phosphorylated pRB. An antibody against actin was used as a loading control.