Figure 4. AA treatment causes a reduction in nestin-positive cells.

E14 cells were differentiated in monolayers for 4 or 6 days in the presence of AA since day 2. Cells were analyzed by cytometry for nestin labeling (A). Nestin positive cells were reduced on treated cultures when evaluated both at day 4 and day 6. Statistically significant differences were found when comparing control and treated cultures for each time-point (B). Data were analyzed by a two-way Anova for independent samples. Error bars = SEM. Statistical significance considered when *p≤ 0.05. Results express at least 3 independent experiments.