Table 1. Multiplex cytological profiling assay components.
Cellular component(s) | Stain | Detection (ex/em) |
---|---|---|
nucleus | Hoechst 33342 | 387/447 nm |
endoplasmic reticulum | concanavalin A (con A) AlexaFluor488 conjugate | 472/520 nm |
nucleoli | SYTO 14 green fluorescent nucleic acid stain | 531/593 nm |
Golgi apparatus and plasma membrane | wheat germ agglutinin (WGA) AlexaFluor594 conjugate | 562/642 nm |
F-actin | phalloidin AlexaFluor594 conjugate | 562/642 nm |
mitochondria | MitoTracker Deep Red | 628/692 nm |