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. 2013 Dec 2;8(12):e80999. doi: 10.1371/journal.pone.0080999

Table 1. Multiplex cytological profiling assay components.

Cellular component(s) Stain Detection (ex/em)
nucleus Hoechst 33342 387/447 nm
endoplasmic reticulum concanavalin A (con A) AlexaFluor488 conjugate 472/520 nm
nucleoli SYTO 14 green fluorescent nucleic acid stain 531/593 nm
Golgi apparatus and plasma membrane wheat germ agglutinin (WGA) AlexaFluor594 conjugate 562/642 nm
F-actin phalloidin AlexaFluor594 conjugate 562/642 nm
mitochondria MitoTracker Deep Red 628/692 nm