Fig. 4.
Identification of ERβ in mitochondrial preparation. (A) MnSOD and histone H1 were used as a mitochondrial and a nuclear marker, respectively. Strong signals for ERβ and MnSOD were evident, but no nuclear (histone H1) signal was seen in the mitochondrial lysate (lane a). In cerebral lysate (lane b), ERβ, MnSOD, and histone H1 were detected. (B) Full-length hrERβ was used as positive control (lane b). A band with the same molecular weight as hrERβ was seen in both human heart homogenate (lane c) and purified human heart mitochondria (lane d). Biotinylated protein standards (lane a) were used as molecular weight markers. Mr values of 80,000, 60,000, and 50,000 are shown.