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MALDI-TOF mass spectrum obtained from the Mr of 50,000–65,000 SDS/PAGE band of the isolated human heart mitochondria after in-gel digestion with trypsin. The major proteins identified by the database search are indicated in the spectrum, and the tryptic peptides that matched to those derived from sequence information of the ER proteins in the National Center for Biotechnology Information database are presented in Tables 1 and 2.
