Table 1. MS identification of ERβ from purified mitochondrial protein.
| m/z submitted | M + H+ matched | ΔMr | Peptide sequence |
|---|---|---|---|
| 993.5115 | 993.4722 | 0.039 | (147-154) SCQACRLR, cysteine alkylation |
| 1165.5804 | 1165.6329 | -0.053 | (59-68) SLEHTLPVNR |
| 1189.6725 | 1189.5449 | 0.13 | (467-477) SKEGSQNPQSQ |
| 1215.6828 | 1215.5428 | 0.14 | (181-191) SADEQLHCAGK, cysteine alkylation |
| 1260.6996 | 1260.6775 | 0.022 | (339-348) LQHKEYLCVK |
| 1442.7152 | 1442.6810 | 0.034 | (179-191) QRSADEQLHCAGK |
| 1922.0015 | 1921.9078 | 0.094 | (1-18) MNYSIPSNVTNLEGGPGR, ox. methionine |
Peptide fragments listed match the National Center for Biotechnology Information (NCBI) nonredundant (nr) database record numbers gi_7441774 and gi_1518263 for ERβ; Mr = 53,384.