Table 2. MS identification of ERβ3 from purified mitochondrial protein.
| m/z submitted | M + H+ matched | ΔMr | Peptide sequence |
|---|---|---|---|
| 993.5115 | 993.4722 | 0.039 | (147-154) SCQACRLR, cysteine alkylation |
| 1122.5952 | 1122.5002 | 0.095 | (504-512) SFEACQQPR, cysteine alkylation |
| 1165.5804 | 1165.6329 | -0.053 | (59-68) SLEHTLPVNR |
| 1194.5684 | 1194.5213 | 0.047 | (467-477) SFEACQQPRE |
| 1215.6828 | 1215.5428 | 0.14 | (181-191) SADEQLHCAGK, cysteine alkylation |
| 1260.6996 | 1260.6775 | 0.022 | (339-348) LQHKEYLCVK |
| 1442.7152 | 1442.6810 | 0.034 | (179-191) QRSADEQLHCAGK |
| 1575.8009 | 1575.7888 | 0.012 | (477-489) LFMLREASCHGVR, cysteine alkylation |
Peptide fragments listed match the National Center for Biotechnology Information (NCBI) nonredundant (nr) database record number gi_3091286 for ERβ3; Mr = 57,519.