Figure 6.

Representative pictures of immunofluorescent labeling for TLR4 and IBa-1 in the far-away hematoma at 6 h, 3 days, and 14 days post injury. Note that IBa-1 positive cells are small and scattered in the area adjacent to the hematoma at 6 h (A, E, I) and appear in the hematoma at 3 days post injury (B, F, J). At 14 days post injury (C, G, K), TLR4 immunoreactive product is seen in the area of the hematoma, and abundant IBa-1 positive cells are large and phagocytotic and with TLR4 immunoreactivity inside the cytoplasm. All of these observation is similar to the lesion site at 3 days post injury (D, H, L), and the stack of scanning pictures of confocal microscopy confirmed that TLR4 immunoreactive product was located in the cytoplasma of IBa-1 positive cells (L). TLR4 immunoreactivity: red; IBa-1 immunoreactivity: green; Hoechest 33342, blue. L, lesion site; H, hematoma. Bar = 50 μm.