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. 2013 Sep 10;13:128. doi: 10.1186/1471-2229-13-128

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Copyright © 2013 Ghosh et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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BbKst protein after purification. A. BbKst-kinase expression in bacterial cells, Lane 1. Total protein in soluble fraction, Lane 2. Total protein in insoluble fraction and Lane 3. Purified protein isolated from the soluble fraction. M = molecular weight marker. B. Western blot of the purified protein against anti-his antibody.