Table 2. Primary antibodies used in this study.
Primary antibody | Type (clone) | Pre-treatment | dilution | Source |
MUC1 | Mouse mono (DF3) | - | 1: 50 | Toray-Fuji bionics (Tokyo, Japan) |
MUC2 | Mouse mono (Ccp58) | MW-CB | 1∶100 | NovoCastra (New Castle, UK) |
MUC5AC | Mouse mono (CLH2) | MW-CB | 1∶200 | NovoCastra (New Castle, UK) |
MUC5B | Goat-poly | MW-CB | 1∶50 | Santa-Cruz (Santa Cruz, CA) |
MUC6 | Mouse mono (CLH5) | MW-CB | 1∶200 | NovoCastra (New Castle, UK) |
mono, monoclonal antibody; poly, polyclonal antibody; MW, microwave treatment; CB, 0.05 M citrate buffer (pH 6);