Fig. 2.
In vivo persistence of H-2Kb clones and in vivo expression of sIL-1ra. Genomic smcy PCR was used to detect SkM at 1 and 3 weeks (n = 4 for each time point). A standard curve (A) was constructed in duplicate by using known numbers of cells [B; cell number in millions (m)]. Enhanced SkM numbers are suggested at both 1 and 3 weeks in the sIL-1ra-SkM group (C). (D) RT-PCR confirmed in vivo expression of sIL-1ra in sIL-1ra-SkM-engrafted hearts (S) at 3 weeks, although not in the cont-SkM (Co) or MI-only groups (MI). RT, reverse transcriptase; W, water; P, 10 ng pcDNA3.1+-hsIL-1ra plasmid.