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Butyrate tolerance level of engineered E. coli. The MG1655 strain containing pQE-adhE2/ptb/buk plasmid was grown under anaerobic condition and resuspended in TB medium containing various concentration of butyric acid and 100 mM glycerol to achieve OD600 of either 1 (A) or 5 (B). The butanol production and cell density were monitored after 120 h of growth in the sealed bottle under anaerobic condition.
