Figure 6.

CD40 binding enhances caspase activation by scFv:G28 fusion proteins of TRAILR1- and TRAILR2-specific TRAIL mutants. (a) HeLa and HT1080 cells along with the corresponding CD40 transfectants were incubated for 5 h with the various scFv:G28-TRAIL variants (10 ng/ml) as indicated. Total cell lysates were analyzed by western blot for the presence of the indicated proteins. (b) OVCAR3 cells were stimulated with 10 ng/ml of the various scFv:G28-TRAIL variants in the presence and absence of scFv:G28-Fc (30 min pretreatment). Cells were analyzed for caspase processing after 5 h