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scFv:G28-TRAIL stimulate CD40 signaling. (a) HeLa cells and HeLa-CD40 transfectants were challenged in triplicates with scFv:G28-TRAIL and Killer-TRAIL and after 8 h supernatants were analyzed by ELISA for the presence of IL-8. (b) HeLa-CD40 transfectants were sensitized for apoptosis induction with CHX (2.5 μg/ml) and otherwise treated as in a. Cell viability was determined next day by crystal violet staining
