Figure 4.

miR-24 transgenic mice show a significant reduction of the proliferation potential in hair follicles. (a) miR-24 overexpression reduces BrdU incorporation in ex vivo primary keratinocytes (*P<0.05). (c and b) Ectopic expression of miR-24 reduced BrdU incorporation in P3 mouse hair follicle. Bar indicates 50 μm. Histograms show quantification of BrdU incorporation (*P<0.05). (d) Skin from transgenic mice showed reduced Ki67-positive cells. Bar indicates 50 μm. (e) Co-immunostaining for Ki67 (green) and AE13 (red) showed altered expression of differentiation markers of hair follicle. Mtx: Matrix. Bar indicates 40 μm