Figure 2.

Effects of single miRNAs or combinations of miRNAs on hMSCs. (A) The relative expression of miRNA in hMSCs transfected with single miRNA mimics analyzed by qRT-PCR at day 6. (B) Albumin expression in hMSCs transfected with a single miRNA mimic for 6 days was analyzed by qRT-PCR. (C) Ectopic miRNA expression in hMSCs transfected with seven miRNA mimics in combination was analyzed by qRT-PCR at day 6. (D) Albumin expression in hMSCs transfected with seven-miRNA mimics for 6 days was analyzed by qRT-PCR. (E) Morphology of hMSCs before and after miRNA transfection for 6 days. (a) hMSC; (b) hMSC treated with seven-miRNA; (c) hMSC treated with mimic-nc; (d) hMSC treated with hepatic differentiation medium. The data represent means±standard deviation (n=3) (A–D). *compared with mimics-nc, P<0.05 by Student's t-test, (A–D)