Figure 3.

The seven-miRNA combination can convert hMSCs into mature functional hepatocytes in vitro. Hepatic function analysis of hMSCs before and after seven-miRNA co-transfection for 6 days. (a) Hepatocyte-specific gene expression analyzed by qRT-PCR. The data represent means±standard deviation (n=3). *Compared with mimics-nc, P<0.05 by Student's t-test. (b) Hepatocyte-specific gene expression analyzed by western blot. (c) Analysis of the BUN synthetic ability of hMSC mediated by the seven-miRNA combination. (d) PAS staining of hMSC mediated by the seven-miRNA combination. (e) ICG uptake analysis of hMSC mediated by the seven-miRNA combination. (f) Analysis of the LDL uptake ability of hMSC mediated by the seven-miRNA combination