Figure 2.

Aβ₄₂ alters morphology and differentiation capacity of NSPCs. Either in the absence or presence of 10 μM Aβ₄₂ for 5 days, the morphology of neurospheres (a) or monolayer cells (b) is observed. (c) Either in the absence or presence of 10 μM Aβ₄₂, NSPCs were differentiation for 5 days, and then were immunostained for neurons (red, β-III-tubulin) and astrocytes (green, GFAP). (d) Untreated and 10 μM Aβ₄₂-treated NSPCs were dissociated to single cells and then at the same density to be grown onto glass coverslips coated with PDL in differentiation condition for 5 days, and stained for lineage markers as above. Quantification analyzed the percentage of β-III-tubulin or GFAP-positive cells. The mean±S.D. of three independent experiments is shown. *P<0.05, **P<0.01 compared with control. Scale bars=100 μm