Figure 3.

Aβ₄₂ promotes NSPCs senescence. (a) NSPCs were treated with Aβ₄₂ (0, 2.5, 5 or 10 μM) for 5 days, and then detected by SA-β-gal staining. The senescent NSPCs were observed by blue cells staining visualized under an inverted microscope. Pictures were acquired and analyzed using the NIS Nikon software. (b) The percentage of SA-β-gal-positive cells was quantified by counting 10² cells on six separate fields for each condition. (c) Immunoblot for levels of p16 and pRb expressions in NSPCs treated with different concentrations of Aβ₄₂. (d) Quantification by densitometric scanning was presented, and results were normalized to the untreated condition. The mean±S.D. of three independent experiments is shown. *P<0.05, **P<0.01 compared with untreated NSPCs. Scale bar=100 μm