Figure 6.

ROS-P38 MAPK signaling pathway is involved in the Aβ₄₂-induced NSPCs senescence via FPR2. NSPCs were pretreated with 10 μM WRW4, 1 mM NAC or 10 μM SB203580 for 1 h before exposure to 10 μM Aβ₄₂. After 5 days, the following were assessed: (a) Intracellular ROS levels in all groups were measured by DCFH-DA; (b) The expressions of p-p38 and p38 proteins in all groups were detected by immunoblot and results were normalized to the untreated condition. (c) The senescent NSPCs were observed by SA-β-gal staining in all groups and statistically analyzed as above. (d) The expressions of pRb and p16 proteins in all groups were determined by western blotting. GAPDH was selected as an inner standard. Quantification by densitometric scanning was presented. Results were normalized to the untreated condition. The mean±S.D. of three independent experiments is shown. *P<0.05, **P<0.01 compared with untreated NSPCs; ^#P<0.05, ^##P<0.01 compared with Aβ₄₂-treated NSPCs. Scale bar=100 μm