Table 3. Accumulation of ppGpp in pea after plant hormone treatments.
| Treatment | ppGpp, pmol/g |
|---|---|
| Water (1 h) (control) | 8.7 ± 3.3 |
| JA (1 h) | 31.2 ± 8.6 |
| JA + cycloheximide (1 h)* | 4.1 ± 0.6 |
| ET (1 h) | 26.3 ± 4.5 |
| ET (2 h) | 19.3 ± 1.7 |
| ABA (1 h) | 40.0 ± 7.0 |
| ABA (2 h) | 14.6 ± 1.5 |
| IAA (1 h) | 6.3 ± 2.5 |
| IAA (2 h) | 7.7 ± 0.9 |
| JA + IAA (1 h) | 8.4 ± 2.0 |
| JA + IAA (2 h) | 12.1 ± 2.5 |
| JA + IAA (1 h)† | 7.4 ± 1.3 |
| ABA + IAA (1 h) | 6.0 ± 0.2 |
| ET + IAA (1 h) | 11.5 ± 1.5 |
Each treatment was done at 25°C under light. Details of each treatment are described in Materials and Methods. The values are means ± SD of three separate experiments. JA, jasmonic acid; ET, ethylene; ABA, abscisic acid; IAA, indole-3-acetic acid.
*
The pea shoots were pretreated with cycloheximide (0.5 mM) for 30 min before treatment with jasmonic acid/cycloheximide solution
†
Instead of 0.1 mM indole-3-acetic acid, 0.02 mM was used