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. 2013 Sep 8;12:78. doi: 10.1186/1475-2859-12-78

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Copyright © 2013 Zhou et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The construction of expression plasmid pBPS-man23. MLS: multiple clone sites; cat-5: chloramphenicol resistance gene; Em^r: erythromycin resistance gene; ori: replication of E. coli; rep: replication of B. brevis; PS: decoding gene of the promoter and signal peptide of B. brevis.