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Comparison of the production yield and mannanase activity produced by different expression plasmids
| Expression plasmid | The amount of total protein(mg/ml) | The amount of mannanase Man23(mg/ml) | Mannanase activity(U/mg) |
|---|---|---|---|
| Wild-type |
8.0 ± 0.06 |
0.72 ± 0.02 |
188.5 ± 0.3 |
| pHY-p43-man23 |
9.5 ± 0.05 |
8.1 ± 0.05 |
222.5 ± 0.2 |
| pBPS-man23 | 8.3 ± 0.05 | 5.8 ± 0.03 | 195 ± 0.2 |
