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Myocardial cell apoptosis assessed by TUNEL staining and cardiac caspase-3 protein expression before and after myocardial ischemia reperfusion. A. Myocardial cell apoptosis assessed by TUNEL, TUNEL positive cells were stained brown as indicated by arrow, B. Cardiac caspase-3 protein expression. Data are expressed as mean ± SEM (n = 7 per group). *P < 0.05, **P < 0.01 vs. C.
