Figure 2.

Effect of AtTCTP on tunicamycin induced cell death. Cell death quantification of tobacco leaf discs transiently over-expressing TCTP of Arabidopsis thaliana (AtTCTP). A shows ion leakage in response to 2.5 μg · ml^-1 tunicamycin, calculated relative to time point T₀. Statistical significances were calculated using analysis of variance (P < 0.05; a indicates wildtype (wt) vs. AtTCTP over-expression). The panel on the left side of B displays tobacco leaf discs floated on 2.5 μg · ml^-1 tunicamycin for 69 hours, either over-expressing AtTCTP or wildtype tobacco (wt). The panel on the right side shows trypan blue staining of dead cells of leaf discs. C shows western blot analysis of leaf discs expressing AtTCTP over time (2, 21, 27, 45, 54, and 69 hours of disc floating).