Figure 6.

mIno80 deficiency inhibits cellular proliferation and anchorage-independent growth in oncogene-transformed cells. (A) Growth curve of SV40^LT-transformed MEFs of the indicated genotypes with or without Ad-Cre expression. Three independent cell lines per genotype were examined. (B, C) Representative colony forming assays for CAG-CreER; mIno80^+/+ or CAG-CreER; mIno80^Δ/Δ MEFs. Colonies were stained with crystal violet and scored. (D, E) Quantification of colonies in B, C. Error bars represent s.e.m. and asterisks indicate statistically significant differences (^*P < 0.05; ^***P < 0.001, Student's t-test). (F) Representative images of colony growth in soft agar (arrows) from SV40^LT/H-Ras^V12-transformed CAG-CreER; mIno80^F/F MEFs with or without 4-HT treatment. (G) Quantification of F. Error bars represent s.e.m. and asterisks indicate statistically significant differences (^***P < 0.001, Student's t-test).