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GtfA transfers l-4-epi-vancosamine to DVV 5 to yield chloroorienticin B 6.(A) Schematic representation of the conversion of 5 to 6.(B) Formation of 6 over 20 min analyzed by analytical RP-HPLC. (C) Michaelis–Menten plot for the measurement of the kinetic parameters of GtfA by varying dTDP-l-epi-vancosamine concentration. The DVV concentration was maintained at 500 μM.
