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irf7 knockdown can restore the expression of the HSC gene runx1 and T-cell gene rag1 in miR-142a-3p morphants. (A) irf7 knockdown restores the expression of HSC genes runx1 (24 hpf) and cmyb (36 hpf), and the T-cell marker rag1 (4 dpf) in miR-142a-3p morphants, as determined by WISH. (B) irf7 knockdown restores runx1 expression at the protein level, as shown by western blotting (left panel) and quantitative analysis of the western blotting results (right panel).
