Figure 2.

Expression of KAR subunit mRNAs in 46C ESCs, NEPs, and NSCs normalized to the expression of the housekeeping gene β-actin (2^ΔCt). (A) GluK1. (B) GluK2. (C) GluK3. (D) GluK4. (E) GluK5. ESCs express KAR subunits at the RNA level; the most abundantly expressed KAR subunit in ESCs and NEPs is GluK3. The expression of all KAR subunits is upregulated upon differentiation into NEPs and subsequently downregulated in NSCs. Data represent mean ± s.e.m.; statistical significances were assigned by One-Way ANOVA followed by Tukey's multiple comparison post-hoc test; ^*p < 0.05; ^**p < 0.01; ^***p < 0.001; n = 3–11 independent experiments. Note that statistical differences in expression of receptor subunits were only calculated between ESCs/NEPs, and NEPs/NSCs, respectively, not between ESCs/NSCs, since ESCs cannot differentiate into NSCs without differentiating into NEPs first.