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Strategy. (A)(Upper Right) PAP heterodimer in which GLD-2 contains the active site and GLD-3 provides RNA-binding specificity. (Lower Right) MS2 coat protein was joined to GLD-2 to recruit it to an RNA. (B) Protocol. MS2/GLD2 protein is expressed in Xenopus oocytes; then two reporter mRNAs are injected. Luciferase mRNA contains MS2 binding sites; β-galactosidase mRNA does not.
