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. Author manuscript; available in PMC: 2013 Dec 3.
Published in final edited form as: Science. 2012 Dec 6;339(6118):10.1126/science.1229277. doi: 10.1126/science.1229277

Figure 2. 5mC erasure is coupled to 5hmC conversion.

Figure 2

(A) Enrichment of 5mC and 5hmC in E10.5-E13.5 PGCs and Epiblast stem cells (EpiSC) over internal exons. (B) 5mC and 5hmC distribution relative to a metagene. (C) Profiles of 5mC (blue) and 5hmC (red) at the Dazl promoter. (D) Glucosyltransferase-qPCR showing quantitative levels of 5mC and 5hmC at a CCGG site in the Dazl promoter. Error bars represent S.E.M. (E & F) DNA methylation (%) by bisulfite sequencing of uninduced (−DOX) or induced (+DOX) Tet1/Tet2 miR or non-targeting (NT) miR PGCLCs at (E) gene promoters and (F) repeat elements. Open and closed circles represent unmethylated and methylated CpGs, respectively. (G) DNA methylation in PGCLCs stably expressing catalytically active (WT) or mutant (Mut) TET1 and TET2. Error bars represent S.E.M of allelic methylation.