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. Author manuscript; available in PMC: 2014 Jun 2.
Published in final edited form as: Nat Commun. 2013 Dec 2;4:10.1038/ncomms3856. doi: 10.1038/ncomms3856

Figure 2. Protochlamydia synthesize purifiable sacculi that contain peptidoglycan.

Figure 2

Cryoprojections (A) and tomographic slices (B-E) through sacculi-like structures purified from Protochlamydia cells. We were unable to obtain similar structures from Simkania. Sacculi had one or two layers (arrowheads) plus short high-density filaments (arrows) on the outside (C enlarged in D, E). Sacculi were digested, reduced and separated by high-pressure liquid chromatography (F). Mass spectrometry analysis of peaks 1-3 (neutral masses indicated in Da) indicated the presence of modified peptidoglycan (see also Table 1 and Supplementary Fig. S1). Bars, 100 nm.