Component	Volume	i.e. Volume for 2 μg input DNA
P1 (ds) Adapter (500 pmol μl−1)	As determined above	1.8 μl
P2 (ds) Adapter (500 pmol μl−1)	As determined above	1.8 μl
10× End-it Buffer	2 μl	2 μl
End-it ATP (10 mM)	2 μl	2 μl
Quick Ligase Enzyme (NEB)	8 μl	8 μl
End-repaired DNA	140 μl	140 μl
Nuclease-Free Water	Variable	4.4 μl
Total volume	160 μl	160 μl