| Final concentration | ×1 volume | |
|---|---|---|
| 2× SYBR Green Master Mix (ABI) | 1× | 15 μl |
| qPCR primer Fw (50 μM) | 0.9 μM | 0.54 μl |
| qPCR primer Rev (50 μM) | 0.9 μM | 0.54 μl |
| H2O | 8.92 μl | |
| Total volume | 25 μl |
*
ROX already pre-mixed in Master Mix
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