Component	Stock concentration	Final concentration	×1 volume	×11 volume
10X Ex Taq Buffer (with MgCl2)	10×	1×	7.6 μl	83.6 μl
2.5 mM each dNTP	2.5 mM	0.25 mM	7.6 μl	83.6 μl
UP2 primer (100 μM)	100 μM	1 μM	0.76 μl	8.36 μl
Nuclease-free water	—	—	59.28 μl	652.08 μl
TaKaRa Ex Taq™ HS	5 U μl−1	0.05 U μl−1	0.76 μl	8.36 μl
Total volume	—	—	76 μl	836 μl