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. Author manuscript; available in PMC: 2013 Dec 3.
Published in final edited form as: Nature. 2008 Mar 19;452(7189):10.1038/nature06714. doi: 10.1038/nature06714

Figure 1. Following the entry of PGCs into the gonads, germ cell chromatin undergoes rapid conformational changes.

Figure 1

A) Dissapearance of linker histone H1 in PGCs at E11.5. PGC7/Stella (in red) was used as germ cell specific marker. PGCs are depicted by arrows. B) Increase in the nuclear size and disappearance of chromocenters in PGCs at E11.5 as observed by DAPI staining of cell suspension from genital ridges. PGCs are depicted by arrows. The lower panel shows identification of PGCs by staining with germ cell specific marker (SSEA1 or Oct4). Scale bars: 10μm. C) Measurement of the nuclear size on the cryosections from E10.5-E13.5 genital ridges shows transient change in the size of germ cell nuclei. The increase is highly statistically significant using t-test. The measured PGC nuclei were identified on the cryosections using Oct4 stainings (data not shown).