Table 3. Isotope enrichment factors determined by MS for the nonsaponifiable lipids isolated from R. setigera grown in the presence of isotopically enriched (13C 20%) substrates.
| Control | Acetate | CO2 | Glucose | Leucine | Deoxyxylulose* | |
|---|---|---|---|---|---|---|
| Phytol (II) | 1.0 (0.9)† | 1.2 | 6.1 | 1.4 | — | 14.2 (26.0)† |
| C25:3 (III) | 1.1 | 4.5 | 5.1 | 1.1 | 1.0 | 0.8 |
| C25:4 (IV) | 1.2 | 4.8 | 5.6 | 1.0 | 1.0 | — |
| C25:5 (V) | 1.0 | 4.3 | 5.5 | 0.9 | — | — |
| C30:5 (VII) | 0.9 | 3.9 | 4.9 | 1.0 | 1.0 | 1.0 |
| C30:5 (VIII) | 0.9 | 3.9 | 5.4 | 0.8 | 0.9 | 1.0 |
| C30:6 (IX) | 1.1 | 4.1 | 4.7 | 0.9 | 0.5 | — |
| C30:6 (X) | 0.9 | 3.9 | 5.4 | 1.0 | 0.4 | — |
| Desmosterol (XI) | 1.3 | 4.6 | 5.5 | 1.2 | 1.3 | 0.8 (1.1)† |
| C25:5 (VI) | 0.9† | 4.5† | — | — | — | 0.9† |
*
Deoxyxylulose was used with 100% 2H enrichment.
†
Strain RS 02/A, all other entries, strain RS 99.