Table 5. 13C NMR analysis of C25 alkenes (III and VI) isolated from R. setigera grown in the presence of 13C-labeled acetate.
| C25 (III)
|
C25 (VI)
|
||||
|---|---|---|---|---|---|
| δ ppm | Carbon | [13C 100%] | δ ppm | Carbon | [13C 20%] |
| 144.7 | 23* | 1.0 | 145 | 23 | 1.6 |
| 142.8 | 7 | — | 136.7 | 6 | 0.9 |
| 136 | 10 | 5.8 | 135 | 10 | 0.9 |
| 123.2 | 9 | 1.4 | 131.3 | 2,14 | 1.0 |
| 122.8 | 20 | 5.2 | 124.6 | 3 | 1.1 |
| 112.2 | 24 | 6.2 | 124.5 | 5 | 1.4 |
| 40.1 | 11 | 0.8 | 123.6 | 9,13 | 1.4 |
| 39.4 | 3 | 0.8 | 112.4 | 24* | 1.0 |
| 38.8 | 13 | 0.7 | 49.2 | 7 | 1.3 |
| 38.3 | 22 | 7.1 | 39.9 | 11 | 1.3 |
| 35.4 | 5 | 0.8 | 37.9 | 22 | 0.9 |
| 34.5 | 21 | 0.7 | 34.5 | 21 | 1.5 |
| 34.4 | 6 | 6.6 | 32.4 | 8 | 0.9 |
| 29.3 | 8 | 5.4 | 30.3 | 20 | 0.9 |
| 28 | 2,14 | 7.5 | 26.9 | 4,12 | 0.9 |
| 25.9 | 12 | 6.3 | 25.8 | 1,15 | 1.6 |
| 25.8 | 4 | 7.5 | 20.6 | 25 | 1.4 |
| 22.7 | 1,15,16,19 | 0.8 | 17.8 | 16,19 | 1.5 |
| 19.6 | 17,25 | 0.9 | 16.3 | 18 | 1.3 |
| 15.8 | 18 | 0.5 | 12 | 17 | 1.5 |
C25:3 (III) was isolated from strain RS 99 ([1-13C]acetate) and C25:5 (VI) was isolated from strain RS 02/A ([2-13C]acetate). Entries correspond to relative enhancements in peak intensities compared with the unlabeled compound. Carbon atoms that should be labeled according to the MVA pathway are indicated in bold.
*
Reference carbon corresponding to C-2 (III) or C-1 (VI) of IDP, unlabeled by either MVA or MEP pathway