Table 3.

Strains and plasmids used in this study

Strains and plasmids	Description	Source or reference
Strains
M. gryphiswaldense MSR-1	wild-type, Nxr	DSM6361
M. gryphiswaldense MSR-1 ΔmamX	mamX deficient mutant, Nxr Gmr	present study
M. gryphiswaldense MSR-1 CmamX	complementation of ΔmamX, NxrGmrTcr	present study
E. coli DH5α	endA1 hsdR17 (r- m+) supE44 thi-1 recA1 gyrA (NalR) recA1 Δ (lacZYA-argF)U169 deoR [Ø80ΔdlacZ ΔM15]	[35]
E. coli S17-1	thi endA recA hsdR with RP4-2-Tc::Mu-Km::Tn7 integrated in chromosome, Smr	[36]
Plasmids
pUCGm	pUC1918 carrying the aacC1 gene, Gmr	[37]
pSUP202	suicide vector for M. gryphiswaldense MSR-1, CmrTcr Ampr	[38]
pSUPpX2	pSUP202 derivative for mamX deletion, GmrCmrAmpr	present study
pRK415	Cloning vector, pRK290 derivative, Tcr	[39]
pRK415X	pRK415 derivative for mamX expression, Tcr	present study