Table 2. Testis weights and number, mobility, and shapes of epididymal spermatozoa in SRC-1 and TIF2 single and compound mutants at 5 and 9 weeks of age.
| WT | TIF2-/- | SRC-1+/-/TIF2-/- | SRC1-/-/TIF2+/- | |
|---|---|---|---|---|
| 5 weeks | ||||
| TW/BW × 103 | 4.1 ± 0.2 (5) | 3.1 ± 0.2** (5) | 2.7 ± 0.8** (5) | 4.4 ± 0.7 (5) |
| 9 weeks | ||||
| TW/BW × 103 | 4.2 ± 0.6 (5) | 2.6 ± 0.7* (5) | 2.6 ± 0.1** (5) | 4.1 ± 0.5 (5) |
| SN × 107 | 6.81 ± 2.57 (4) | 3.40 ± 1.68 (3) | 0.06 ± 0.04** (3) | 3.34 ± 0.74 (3) |
| SM, % | 57.8 ± 6.4 (4) | 17.0 ± 5.7** (3) | 13.8 ± 4.3** (3) | 49.1 ± 5.9 (3) |
| AS, % | 13.4 ± 3.0 (4) | 46.5 ± 3.2** (3) | 59.4 ± 5.1** (3) | 9.5 ± 2.6 (3) |
TW, testis weight; BW, body weight; SN, number of spermatozoa per ml of cauda epididymal fluid; SM, percentage of mobile spermatozoa; AS, percentage of abnormal spermatozoa displaying looped tails and/or triangular head shapes. The number of mice that were analyzed is indicated in parentheses. Note that no significant changes in number and morphology of spermatozoa were observed in SRC-1-/-/TIF2+/- mice and that, although the number of spermatozoa in TIF2-/- epididymides was apparently lower than in their WT counterparts, this difference was not statistically significant. *, P < 0.05 vs. WT in unpaired t test. **, P < 0.01 vs. WT in unpaired t test.