Fig. 2.

Mitotic abnormalities in the absence of MAD2. (A) Time-lapse photography of lamin and MAD2 knockdown HeLa cells (×40). (Upper) A lamin-transfected cell entering mitosis at 30 min and completing cytokinesis at 2 h. (Lower) The MAD2 knockdown cell enters mitosis at 50 min, sheds cellular debris caused by the abnormal mitosis (arrow, 1:20 min), and exits at 1 h and 50 min as a multinucleated cell (best seen at arrow, 2:20 min). (B and C). Nomarsky differential interference contrast microscopy images of a multinucleated MAD2 knockdown HaCaT cell stained with DAPI (×63).