Figure 4.

Comparison of ET- and BT-Stx2dact cytotoxicity for Vero cells treated with an inhibitor of GSLs or an enhancer of Gb3 expression. Cells were incubated with media alone or PPMP (A), a compound that decreases expression of GSLs, including Gb3, or butyrate (B), a short-chain fatty acid that increases expression of Gb3, and then either ET- or BT-Stx2dact was added. The higher the A₆₃₀, the more fixed and crystal violet-stained cells remain after toxin exposure. Representative data are presented from a total of six experiments.