Table 3.
Primer sequences used to amplify antioxidant and phase II enzymes in liver and kidney of control and OA-treated mice.
| TARGET cDNA | GeneBank accession | PRIMER SEQUENCE |
|---|---|---|
| CAT-catalase | NM_009804.2 | 5' GCTGAGAAGCCTAAGAACGCAAT 3' |
| 5' CCCTTCGCAGCCATGTG 3' | ||
| SOD1-superoxide dismutase 1, soluble | NM_011434.1 | 5' CCAGTGCAGGACCTCATTTTAAT 3' |
| 5' TCTCCAACATGCCTCTCTTCATC 3' | ||
| NQO1-NAD(P)H dehydrogenase, quinone 1 | NM_008706.5 | 5' GGTTTACAGCATTGGCCACACT 3' |
| 5' TTCCAGACGTTTCTTCCATCCT 3' | ||
| NQO2-NAD(P)H dehydrogenase, quinone 2 | NM_020282.3 | 5' GTTCACACTCAGCTTCTCTTGCA 3' |
| 5' GGACACAATACCCAAAGGGAAA 3' | ||
| HMOX1-heme oxygenase (decycling) 1 | NM_010442.2 | 5' CCTCACTGGCAGGAAATCATC 3' |
| 5' CCTCGTGGAGACGCTTTACATA 3' | ||
| HMOX2-heme oxygenase (decycling) 2 | NM_010443.2 | 5' CCAATTCTACCTGTTTGAGCATGT 3' |
| 5' AAATTCAGGTCCAAGGCATTCA 3' | ||
| GPX1-glutathione peroxidase 1 | NM_008160.5 | 5' CCCGTGCGCAGGTACAG 3' |
| 5' CAGCAGGGTTTCTATGTCAGGTT 3' | ||
| GPX2-glutathione peroxidase 2 (gastrointestinal) | NM_030677.2 | 5' GCTGCCCTACCCTTATGATGAC 3' |
| 5' GCACGGGACTCCATATGATGA 3' | ||
| Nrf2-nuclear factor erythroid-derived 2-like 2 | NM_010902.3 | 5'-CCGAGATATACGCAGGAGAGGTA-3' |
| 5'-GCTCGACAATGTTCTCCAGCTT-3' | ||
| PPIA-peptidylprolyl isomerase A (cyclophilin A) | NM_008907.1 | 5' CAAATGCTGGACCAAACACAA 3' |
| 5' GCCATCCAGCCATTCAGTCT 3' |