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Relative invasion frequency (mean ± SD of three separate experiments in triplicate) of meningitis-causing E. coli strain RS218 and its CNF1 deletion mutant in human brain mircovascular endothelial cells (HBMEC) monolayer. The ΔCNF1 mutant was significantly defective (*p < 0.02 compared to the parent strain RS218, which was set at 100%) in HBMEC invasion.
