Table 3. Propagation of trunk NC precursors.
| % of total clones (no. of cells per clone)
|
||||||
|---|---|---|---|---|---|---|
| Control
|
ET3
|
|||||
| Colony type | I | I | II | III | IV | V |
| G | 29.5 (15) | 13 (30) | 20 (30) | 20.5 (30) | 23 (5) | 0 |
| M | 23.5 (8) | 4.5 (40) | 18* (30) | 22 (30) | 7‡ (10) | 80 (10) |
| F | 6 (8) | 16 (15) | 5† (5) | 2 (2) | 0 | 0 |
| GM | 17.5 (50) | 45.5 (1500) | 50 (150) | 52 (80) | 66 (30) | 20 (10) |
| GF | 11.5 (30) | 15 (40) | 4.5* (10) | 0 | 0 | 0 |
| GMF | 6 (50) | 3 (2000) | 0 | 0 | 0 | 0 |
| U | 6 (6) | 3 (30) | 2.5 (20) | 3.5 (1) | 4 (2) | 0 |
The types of colony produced by trunk NCC through serial subcloning were quantified as the percentage of total clone number at successive cloning I to V. The mean cell number per clone is indicated in parentheses. In control medium, primary colonies could not be propagated by subcloning (data not shown). In presence of ET3, the number of colonies used for successive subclonings was 13 (cloning I), 10 (cloning II), 6 (cloning III), and 1 (cloning IV). Total numbers of clones are as follows: 17 (for control I) and 68, 195, 174, 112 and 15 (for ET3 I-V, respectively). Values were obtained from six independent experiments. Differences between two subsequent rounds of cloning are indicated. *, P < 0.05; †, P < 0.001; ‡, P < 0.0001.